TTP Labtech, Phenotypic Screening - 7

PHENOTYPIC SCREENING an e-Book Series Perspective

enough for use in high throughput screening campaigns, yet retain
enough biological complexity to make them predictive in vivo models
of tumours. This article discusses the current approaches to develop
HTS-compatible 3D assays to address this need.
3D Cell Cultures as In Vitro Tumour Models
Whilst 2D culture of adherent cells has been the mainstay
in oncology phenotypic screening, it has become clear that 3D
cell cultures are better at mimicking complex cellular systems.
It is known that avascular tumours exhibit many complex
biological features lacking in 2D cell monoculture: they have
metabolic gradients (e.g. for oxygen, carbon dioxide, nutrients,
waste) as well as proliferative and gene expression gradients.
In addition to the cancerous cells they can comprise a range of
other cell types, such as endothelial, stromal and epithelial cells,
which influence tumour growth and survival. It is therefore,
hardly surprising that cancer cell lines cultured in homogenous
2D monolayers (with unlimited access to oxygen and nutrients)
can respond to drug treatments very differently than tumours
in vivo. 3D cell cultures retain many of the phenotypic and
genetic traits of tumours (Table 1), and therefore promise to
deliver more physiologically relevant drug responses
(Case Study 1).
7 | GENengnews.com

2D
3D
Cell Culture Cell Culture

In Vivo

$

$-$$

$$$$$

Amenability to HTS

✓✓✓

✓✓

✗

Multivariate Readout

✓✓✓

✓✓

✗

Native Morphology

✓

✓✓

✓✓✓

Native Cell-to-Cell
Interactions and Signalling

✓

✓✓

✓✓✓

Phenotype Heterogeneity
(e.g. Proliferating, Non-Proliferating,
Necrotic Cells)

✓

✓✓

✓✓✓

Biological Gradients
(e.g. Nutrients, O2, Waste,
Drug Concentration)

✗

✓

✓✓

Cost

Table 1. Bridging the gap between 2D cell cultures and in vivo animal models


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TTP Labtech, Phenotypic Screening

Table of Contents for the Digital Edition of TTP Labtech, Phenotypic Screening

Contents
TTP Labtech, Phenotypic Screening - 1
TTP Labtech, Phenotypic Screening - 2
TTP Labtech, Phenotypic Screening - 3
TTP Labtech, Phenotypic Screening - Contents
TTP Labtech, Phenotypic Screening - 5
TTP Labtech, Phenotypic Screening - 6
TTP Labtech, Phenotypic Screening - 7
TTP Labtech, Phenotypic Screening - 8
TTP Labtech, Phenotypic Screening - 9
TTP Labtech, Phenotypic Screening - 10
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TTP Labtech, Phenotypic Screening - 19
TTP Labtech, Phenotypic Screening - 20
TTP Labtech, Phenotypic Screening - 21
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TTP Labtech, Phenotypic Screening - 24
TTP Labtech, Phenotypic Screening - 25
TTP Labtech, Phenotypic Screening - 26
TTP Labtech, Phenotypic Screening - 27
TTP Labtech, Phenotypic Screening - 28
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