Unchained Labs eBook - 15

Unleash your biologic * Protein characterization turned up to 11

Some applications don't require a temperature ramp, but rather hold a constant
temperature for an incubation period,
during which samples can be continuously
monitored. For example, isothermal stability
experiments can be carried out for hours
or even days, just below the melting temperature of the proteins, to allow for a detailed picture of unfolding and aggregation
events. Similarly, chemical denaturation
experiments can be performed with Uncle,
where quantitative ΔG values are obtained
for proteins at ambient temperatures. A
DLS-based viscosity application allows for
screening of 48 samples at once, adding
viscosity measurements to an earlier stage
in the development process.
In all, eleven different applications can be
performed with one instrument, greatly

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reducing laboratory space requirements
and sample volume requirements. Uncle
creates further efficiency by removing the
need to maintain multiple single-measurement instruments and the necessity
to train scientists on multiple instruments
and software packages. These efficiencies
provide researchers a leg up for quickly
comparing constructs or formulations and
selecting winning biologics. "Unchained
Lab's Uncle system has been a great
addition to our lab" says Tim Myles, Ph.D.,
Director of Protein Science at Aragen
Bioscience. "Estimations of thermostability (Tm) and aggregation (Tagg) can
be performed in multiple buffer conditions,
concentrations, freeze thaws, pH hold
and agitation in less than one day. Such
profiling allows for faster formulation
and bioprocess development studies."

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Unchained Labs eBook

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