Agilent eBook - 11

Best Sizing Practices
with the Agilent 5200 Fragment
Analyzer System


Chava Pocernich, Ben Deist,
Whitney Pike, and Denise Warzak,
Agilent Technologies, Inc.

Quality control checkpoints for sizing are a necessity for many nucleic acid applications
and ensure successful outcomes. The Agilent 5200 Fragment Analyzer system provides
reliable sizing for fragments and smears with Agilent ProSize data analysis software.
This Application Note describes factors that affect sizing and the best practices for
achieving accurate sizing of the sample.
Accurate sizing is essential for quality control analysis of nucleic acids. The Agilent
5200 Fragment Analyzer system offers a wide range of qualitative and quantitative
kits that provide reliable sizing for fragments and smears with ProSize data analysis
software. Standard deviation, precision, and accuracy were the parameters for evaluating sizing. Standard deviation is used to quantify the amount of variation or dispersion
of a set of data values from the mean. Accuracy measures the closeness of a number
to the true value and is reported as % error (Equation 1). Precision is the closeness of
two or more measurements and is independent of accuracy. A low % CV (coefficient
of variance, Equation  2), representing precision, demonstrates the closeness of the
measurements and reliability of the average.
% Error =



Equation 1. Accuracy.

Calculated - Known

× 100

%CV =
Equation 2. Precision.

std dev.

× 100

Additional Content
The Agilent Fragment
Analyzer systems let you
focus on your nucleic acid QC
results while improving your
lab efficiency.


Agilent eBook

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