Shaklee Product Catalogue 2016 - Canada/English - 87


CLINICAL RESEARCH | PROOF OF THE PRODUCTS

Personal Care
Subject: Enfuselle® Product Line (Global Skin
Care Program)
The introduction of the Enfuselle® skin care line has
been accompanied by extensive clinical testing,
outlined below. For more information, contact your
Distributor or Sponsor to obtain a copy of this
information, or visit Shaklee.com.
Some of the Enfuselle® clinical studies utilized
contralateral (half-face) studies. Bioinstruments
were used to objectively measure the depth of
fine lines and wrinkles on one side of the face
where Enfuselle® was being applied and compared
these results to the control side where no skin care
products or makeup were used. The results of the
tests, due in part to the rapidly changing climate
conditions, were extraordinary: where no skin care
or cosmetic products were used, the visible signs
of aging became measurably deeper, while the skin
treated with Enfuselle® products steadily improved.
In addition to bioinstrumentation, subjects
completed a detailed questionnaire following the
tests subjectively rating the products' results.
Subject: Enfuselle Vital Repair+® Complex:
In vitro study of the effectiveness of a unique
combination of known antioxidants, Vital
Repair+®, in human cell cultures
Enfuselle® Vital Repair+® complex: Exhibits
significant antioxidant activity in cell cultures of
human skin cells exposed to ultraviolet (UV) light.
This unique, patented (#6,036,946) combination
of ingredients composed of vitamins A, C, and
E, along with panthenol, grapeseed extract,
superoxide dismutase, and beta glucan, has been
shown to protect against cellular death caused
by exposure to the UV wavelengths of natural
sunlight. The activity of this complex was found to
be synergistic, that is, the activity of the complex
was much greater than the sum of the activity
of the individual components or the sum of the
activity of those component materials.
The effectiveness of Vital Repair+® complex has
been demonstrated in human cell cultures in which
free radicals were generated by exposing those
cultures to ultraviolet (UV) light. When compared
to cell cultures exposed to the same amount of UV
light but not treated with Vital Repair+® complex or
its component ingredients, the cell cultures treated
with Vital Repair+® complex were found to exhibit a
significantly lower number of cell deaths.
All cell culture measurements were made within
48 hours following exposure of the cell cultures
to the UV light. These measurements were run
in triplicate for each material tested and were
conducted using techniques commonly employed
in cell culture technology. The reduction in cell
death was accompanied by an increased number
of living (viable) dividing skin cells in the cultures
treated with Vital Repair+® complex.
Conclusion: The activity of this complex of
antioxidants clearly demonstrates that Vital
Repair+® is effective in blocking the complex
cascade of free radical reactions brought about by
exposure of skin to UV light.
Subject: Antioxidant Activity of Vital Repair+®
Complex From Enfuselle® Formulations When
Applied to Human Skin
Vital Repair+® complex in Enfuselle® Formulations
Provides Antioxidant Activity: A test of three
of the Enfuselle® skin care formulations clearly
demonstrates that each formulation exhibits

significant antioxidant activity when applied to
the surface of intact human skin. The level of
antioxidant activity was found to be significantly
greater than that of control treated skin without
the Vital Repair+® complex. These results clearly
demonstrate that the Vital Repair+® complex
present in the Enfuselle® skin care formulations
exhibits antioxidant activity as demonstrated in the
human skin cell cultures.
The effectiveness of Vital Repair+® complex
antioxidant complex included in the Enfuselle®
skin care products was demonstrated in human
cell cultures. However, this does not unequivocally
ensure this same effectiveness when applied to
human skin. Therefore, a test was conducted
to show this efficacy on human skin. It is well
documented that when beta carotene is exposed to
long wavelength ultraviolet (UVA, 320 to 400 nm)
radiation, as found in sunlight, it undergoes a free
radical reaction during which it changes from an
orange colour to a pale yellow colour. Shaklee
scientists used this knowledge to demonstrate the
effectiveness of Vital Repair+® complex from the
Enfuselle® formulations. Solutions of beta carotene
were applied to the forearms of human subjects
and allowed to dry completely. The Enfuselle®
formulations were then applied to selected sites
on top of the beta carotene treated sites. All test
sites were then exposed to UVA light. The changes
in the colour of the test sites were then monitored
using a Minolta™ Chromameter throughout the
UVA exposure period.
The colour changes in the treated and control
sites were exposed to six (6) different intensities
of UVA light. Based upon the Chromameter data
gathered from this test, the following conclusions
were drawn:
When compared with control test sites not treated
with the Enfuselle® formulations, the Vital Repair+®
complex delivers the following effectiveness
against UVA-induced free radical reactions:
* Up to twice the antioxidant effectiveness at low
UVA doses
* Up to three times the antioxidant effectiveness
at high UVA doses
When compared with cell cultures exposed to UV
light in the absence of Vital Repair+® complex, the
following data were found:
* 40% reduction in the number of cell deaths
Additionally, the reduction in the number of cell
deaths in the cultures treated with Vital Repair+®
complex was greater than any of the individual
components of Vital Repair+® complex tested
separately or the sum of their combined activities.

were only allowed to cleanse the untreated side
with their normal cleanser. No makeup or other
skin care products were worn by the subjects.
Approximately 50% of the panel were contact lens
wearers. The safety of Enfuselle® Eye Treatment
was evaluated by a board-certified dermatologist
and ophthalmologist. They found that under the
conditions of the test, the product was safe for use,
even by contact lens users.
* 120% increase in skin resilience and firmness in
14 days
* 209% decrease in visible fine lines of the skin in
56 days
* 223% decrease in appearance of skin wrinkles in
14 days
* 32% increase in retained skin moisture in 56 days
* A definitive evening of skin tone in 84 days
Conclusion: Enfuselle® Eye Treatment
significantly improves skin firmness and
resilience, and dramatically improves the texture
of eye-area skin while significantly reducing the
visible signs of aging of all skin types.

Enfuselle® Has Been Awarded Seven U.S. Patents,
With One Patent Pending
*	 U.S.	Patent	#6,015,548:	"High-efficiency	skin	
protection formulation with sunscreen agents
and antioxidants."
*	 U.S.	Patent	#6,036,946:	"Methods	for	protecting	
skin from damaging effects of ultraviolet light."
*	 U.S.	Patents	#6,174,519,	#6,235,	272:	
"Composition for protecting skin from
damaging effects of ultraviolet light."
*	 U.S.	Patent	#6,146,664:	"Stable	topical	ascorbic	
acid compositions."
*	 U.S.	Patent	#6,403,110:	"Topical	treatment	for	
oily skin."
*	 U.S.	Patent	#6,723,688:	"Cleanser	and	bar	gentle	
to human skin."
For more clinical data please visit Shaklee.com.
Reference: Data on File, Shaklee Corporation.

Conclusion: This test clearly demonstrates that
Vital Repair+® complex protects the skin from
UVA-induced colour change of beta carotene,
which is known to occur through a free radical
pathway. Therefore, the antioxidant activity of the
Vital Repair+® complex to protect the surface of
human skin against free radical damage has been
demonstrated.
Subject: Enfuselle® Eye Treatment
Enfuselle® Eye Treatment was evaluated under
clinically controlled conditions. Twenty-three
men and women between the ages of 30 and
70 used Enfuselle® Eye Treatment for a period
of 84 days. The product was applied to the skin
around one eye, in a controlled and supervised
manner. The skin around the other eye area
served as the control. The treated and untreated
sides were randomized to avoid bias. Subjects
To order, call your Shaklee Independent Distributor, visit SHAKLEE.CA, or call 1-800-263-6674

87


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Table of Contents for the Digital Edition of Shaklee Product Catalogue 2016 - Canada/English

Shaklee Product Catalogue 2016 - Canada/English
Table of Contents
Healthy Foundations
Healthy Solutions
Healthy Weight
Healthy Home
Healthy Beauty
Clinical Research
Opportunity
Shaklee Product Catalogue 2016 - Canada/English - Shaklee Product Catalogue 2016 - Canada/English
Shaklee Product Catalogue 2016 - Canada/English - Table of Contents
Shaklee Product Catalogue 2016 - Canada/English - 3
Shaklee Product Catalogue 2016 - Canada/English - 4
Shaklee Product Catalogue 2016 - Canada/English - 5
Shaklee Product Catalogue 2016 - Canada/English - 6
Shaklee Product Catalogue 2016 - Canada/English - 7
Shaklee Product Catalogue 2016 - Canada/English - 8
Shaklee Product Catalogue 2016 - Canada/English - 9
Shaklee Product Catalogue 2016 - Canada/English - Healthy Foundations
Shaklee Product Catalogue 2016 - Canada/English - 11
Shaklee Product Catalogue 2016 - Canada/English - 12
Shaklee Product Catalogue 2016 - Canada/English - 13
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Shaklee Product Catalogue 2016 - Canada/English - 36
Shaklee Product Catalogue 2016 - Canada/English - 37
Shaklee Product Catalogue 2016 - Canada/English - Healthy Solutions
Shaklee Product Catalogue 2016 - Canada/English - 39
Shaklee Product Catalogue 2016 - Canada/English - 40
Shaklee Product Catalogue 2016 - Canada/English - 41
Shaklee Product Catalogue 2016 - Canada/English - 42
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Shaklee Product Catalogue 2016 - Canada/English - 51
Shaklee Product Catalogue 2016 - Canada/English - 52
Shaklee Product Catalogue 2016 - Canada/English - 53
Shaklee Product Catalogue 2016 - Canada/English - Healthy Weight
Shaklee Product Catalogue 2016 - Canada/English - 55
Shaklee Product Catalogue 2016 - Canada/English - 56
Shaklee Product Catalogue 2016 - Canada/English - 57
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Shaklee Product Catalogue 2016 - Canada/English - 61
Shaklee Product Catalogue 2016 - Canada/English - Healthy Home
Shaklee Product Catalogue 2016 - Canada/English - 63
Shaklee Product Catalogue 2016 - Canada/English - 64
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Shaklee Product Catalogue 2016 - Canada/English - 71
Shaklee Product Catalogue 2016 - Canada/English - Healthy Beauty
Shaklee Product Catalogue 2016 - Canada/English - 73
Shaklee Product Catalogue 2016 - Canada/English - 74
Shaklee Product Catalogue 2016 - Canada/English - 75
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Shaklee Product Catalogue 2016 - Canada/English - 80
Shaklee Product Catalogue 2016 - Canada/English - 81
Shaklee Product Catalogue 2016 - Canada/English - Clinical Research
Shaklee Product Catalogue 2016 - Canada/English - 83
Shaklee Product Catalogue 2016 - Canada/English - 84
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Shaklee Product Catalogue 2016 - Canada/English - 93
Shaklee Product Catalogue 2016 - Canada/English - Opportunity
Shaklee Product Catalogue 2016 - Canada/English - Cover3
Shaklee Product Catalogue 2016 - Canada/English - Cover4
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