Food Protection Trends - November 2008 - 810

In summary, to ensure food safety and security of globally transported food there is a need for developing science based harmonized microbial testing criteria. While plausible from a scientific standpoint, politically this may be very difficult. Finally, performance based standards may be the fastest route to harmonization. S4 – Bacterial Physiology — A Forgotten theme that is Critical for the Food Microbiologist Diego Paiva, Auburn University and Bala Jagadeesan, Purdue University The symposium focused on the effect of stress and growth environment on bacterial physiology and its implication in food microbiology. The presenters were Charles Kaspar, Kathryn Boor, Roy Betts, Teresa Bergholz, Larry Beuchat and Martin Wiedmann. The complexity of the molecular interactions in regulating the phenotypic expression of the bacteria during growth and stress conditions at the transcriptional, translational and post translational level were discussed in depth along with the role of master regulators such as Histone Nucleoid Structure Proteins (H-NS). Stress response and virulence gene expression regulation by sigma B factor in Listeria monocytogenes was highlighted to emphasize the importance of understanding the role of general stress response factors in the survival and virulence of foodborne pathogens in general – L. monocytogenes in particular. The diverse growth and adaptive response of Salmonella to complex food matrix and growth parameter interactions helped reinforce the fact that each food system is unique and thus the physiological response of the bacteria could considerably vary depending on food. In addition, the species, strain, age of the cells and the culture growth phase play a very important role on metabolism and physiological response of bacterial cells to stressful environments. Another very important factor which affects bacterial survival and growth is the food in question and how this specific food is processed, prepared for consumption and commercialized. Some other factors within food composition such as water activity, pH, nutrient availability and physical state of food (liquid, paste, solid) must also be taken in consideration while preparing the inoculum for challenge studies. The difference between shock response and continuous response was explained in Escherichia coli O157:H7 and its impact in conferring cross protection, resistance to antimicrobials, design of challenge studies and food processing environment were discussed. Finally, the bacterial physiology and its effect on pathogen detection sensitivity using genetic, protein based and traditional approaches were discussed in detail. Specifically with the development of rapid detection methods, the need to understand about the physiological status of the bacteria enriched for a shorter duration was stressed upon. Thus the symposium emphasized the complexity of the molecular networks regulating the bacterial phenotypic expression under different growth and stress conditions. The symposium also focused on the applied aspects of the basic knowledge gained in areas such as design of challenge studies and effect on pathogen detection systems. S5 – Sampling and Sample Prep: Unglamorous but very necessary Heidi Weinkauf, Iowa State University and Michele Yabes-Manuzon, The Ohio State University Sampling and sample preparation are two key steps for the effective detection of microorganisms. However, most of the research to date has focused on developing assay technologies. Nonetheless, without reliable upstream methods, these detection procedures can be ineffective. Mary Lou Tortorello from FDA-CFSAN stressed the need to develop alternative methods without the use of culture enrichment. In order to advance research in this area, a sample prep multi-disciplinary working group was formed in July 2007 to help promote research in sampling and sample preparation methods for rapid food analyses. Byron Brehm-Stecher from Iowa State University discussed what comprises the most effective sample preparation methods. He pointed out that techniques and concepts could be borrowed from both allied and non-related fields to help improve sample prep methods. Improvements in these upstream methods are needed to address the physical challenges posed by the food matrix, and may eventually enable the direct analysis from the food without the isolation of cells. Emilio Esteban of USDA-FSIS-OPHS emphasized that the sampling method of choice depends on the questions that need to be answered. He discussed the basis of designing surveillance and monitoring systems. This requires defining the purpose and most appropriate sample prep procedures, applying the method, analyzing the results and then making recommendations and implementing the ideas. Tony Sharpe of Filtaflex Ltd. gave a historical overview of common approaches to sample preparation. The development of the stomacher in the 1970s was discussed along with alternatives that have since been created. The stomacher is a traditional method that is still widely used, however 810 FOOD PROTECTION TRENDS | NOVEMBER 2008

Food Protection Trends - November 2008

Table of Contents for the Digital Edition of Food Protection Trends - November 2008

Food Protection Trends - November 2008
Contents
Sustaining Members
Perspectives from Your President
Commentary from the Executive Director
Biofilm Formation of Listeria monocytogenes on Various Conveyor Belt Surfaces
Third-Party Certification of Agro-Products in China: A Study of Agro-Product Producers in Guangzhou, Shenzhen, Hangzhou and Qingdao
IAFP 2008 in Review
Award Winners
3M Microbiology - Black Pearl Award Winner
Ivan Parkin Lecture - Russell S. Flowers
John H. Silliker Lecture - Michael P. Doyle
Student Travel Scholarships
Session Summaries
Highlights of the Executive Board Meeting
Minutes of the 95th Annual Business Meeting
Committee Minutes
Executive Board Response to Committee Recommendations
Exhibitors of IAFP 2008
Special Contributors and Sponsors
Call for Awards - IAFP 2009
New Members
What's Happening in Food Safety
Industry Products
Coming Events
Advertising Index
Journal of Food Protection Table of Contents
Audiovisual Library Order Form
Booklet Order Form
Membership Application
Food Protection Trends - November 2008 - Food Protection Trends - November 2008
Food Protection Trends - November 2008 - Cover2
Food Protection Trends - November 2008 - 745
Food Protection Trends - November 2008 - Contents
Food Protection Trends - November 2008 - 747
Food Protection Trends - November 2008 - 748
Food Protection Trends - November 2008 - 749
Food Protection Trends - November 2008 - 750
Food Protection Trends - November 2008 - 751
Food Protection Trends - November 2008 - 752
Food Protection Trends - November 2008 - Sustaining Members
Food Protection Trends - November 2008 - 754
Food Protection Trends - November 2008 - 755
Food Protection Trends - November 2008 - Perspectives from Your President
Food Protection Trends - November 2008 - 757
Food Protection Trends - November 2008 - Commentary from the Executive Director
Food Protection Trends - November 2008 - 759
Food Protection Trends - November 2008 - Biofilm Formation of Listeria monocytogenes on Various Conveyor Belt Surfaces
Food Protection Trends - November 2008 - 761
Food Protection Trends - November 2008 - 762
Food Protection Trends - November 2008 - 763
Food Protection Trends - November 2008 - 764
Food Protection Trends - November 2008 - Third-Party Certification of Agro-Products in China: A Study of Agro-Product Producers in Guangzhou, Shenzhen, Hangzhou and Qingdao
Food Protection Trends - November 2008 - 766
Food Protection Trends - November 2008 - 767
Food Protection Trends - November 2008 - 768
Food Protection Trends - November 2008 - 769
Food Protection Trends - November 2008 - 770
Food Protection Trends - November 2008 - IAFP 2008 in Review
Food Protection Trends - November 2008 - 772
Food Protection Trends - November 2008 - 773
Food Protection Trends - November 2008 - 774
Food Protection Trends - November 2008 - 775
Food Protection Trends - November 2008 - Award Winners
Food Protection Trends - November 2008 - 777
Food Protection Trends - November 2008 - 778
Food Protection Trends - November 2008 - 779
Food Protection Trends - November 2008 - 780
Food Protection Trends - November 2008 - 781
Food Protection Trends - November 2008 - 782
Food Protection Trends - November 2008 - 783
Food Protection Trends - November 2008 - 784
Food Protection Trends - November 2008 - 785
Food Protection Trends - November 2008 - 786
Food Protection Trends - November 2008 - 787
Food Protection Trends - November 2008 - 788
Food Protection Trends - November 2008 - 789
Food Protection Trends - November 2008 - 790
Food Protection Trends - November 2008 - 791
Food Protection Trends - November 2008 - 792
Food Protection Trends - November 2008 - 793
Food Protection Trends - November 2008 - 3M Microbiology - Black Pearl Award Winner
Food Protection Trends - November 2008 - 795
Food Protection Trends - November 2008 - Ivan Parkin Lecture - Russell S. Flowers
Food Protection Trends - November 2008 - 797
Food Protection Trends - November 2008 - 798
Food Protection Trends - November 2008 - 799
Food Protection Trends - November 2008 - John H. Silliker Lecture - Michael P. Doyle
Food Protection Trends - November 2008 - 801
Food Protection Trends - November 2008 - 802
Food Protection Trends - November 2008 - 803
Food Protection Trends - November 2008 - Student Travel Scholarships
Food Protection Trends - November 2008 - 805
Food Protection Trends - November 2008 - 806
Food Protection Trends - November 2008 - 807
Food Protection Trends - November 2008 - Session Summaries
Food Protection Trends - November 2008 - 809
Food Protection Trends - November 2008 - 810
Food Protection Trends - November 2008 - 811
Food Protection Trends - November 2008 - 812
Food Protection Trends - November 2008 - 813
Food Protection Trends - November 2008 - 814
Food Protection Trends - November 2008 - 815
Food Protection Trends - November 2008 - 816
Food Protection Trends - November 2008 - 817
Food Protection Trends - November 2008 - 818
Food Protection Trends - November 2008 - 819
Food Protection Trends - November 2008 - 820
Food Protection Trends - November 2008 - 821
Food Protection Trends - November 2008 - 822
Food Protection Trends - November 2008 - 823
Food Protection Trends - November 2008 - 824
Food Protection Trends - November 2008 - 825
Food Protection Trends - November 2008 - 826
Food Protection Trends - November 2008 - 827
Food Protection Trends - November 2008 - 828
Food Protection Trends - November 2008 - 829
Food Protection Trends - November 2008 - 830
Food Protection Trends - November 2008 - 831
Food Protection Trends - November 2008 - 832
Food Protection Trends - November 2008 - Highlights of the Executive Board Meeting
Food Protection Trends - November 2008 - Minutes of the 95th Annual Business Meeting
Food Protection Trends - November 2008 - 835
Food Protection Trends - November 2008 - Committee Minutes
Food Protection Trends - November 2008 - 837
Food Protection Trends - November 2008 - 838
Food Protection Trends - November 2008 - 839
Food Protection Trends - November 2008 - 840
Food Protection Trends - November 2008 - 841
Food Protection Trends - November 2008 - 842
Food Protection Trends - November 2008 - 843
Food Protection Trends - November 2008 - 844
Food Protection Trends - November 2008 - 845
Food Protection Trends - November 2008 - 846
Food Protection Trends - November 2008 - 847
Food Protection Trends - November 2008 - 848
Food Protection Trends - November 2008 - 849
Food Protection Trends - November 2008 - 850
Food Protection Trends - November 2008 - 851
Food Protection Trends - November 2008 - 852
Food Protection Trends - November 2008 - 853
Food Protection Trends - November 2008 - 854
Food Protection Trends - November 2008 - 855
Food Protection Trends - November 2008 - 856
Food Protection Trends - November 2008 - 857
Food Protection Trends - November 2008 - Executive Board Response to Committee Recommendations
Food Protection Trends - November 2008 - 859
Food Protection Trends - November 2008 - 860
Food Protection Trends - November 2008 - 861
Food Protection Trends - November 2008 - 862
Food Protection Trends - November 2008 - 863
Food Protection Trends - November 2008 - 864
Food Protection Trends - November 2008 - 865
Food Protection Trends - November 2008 - Exhibitors of IAFP 2008
Food Protection Trends - November 2008 - 867
Food Protection Trends - November 2008 - 868
Food Protection Trends - November 2008 - 869
Food Protection Trends - November 2008 - 870
Food Protection Trends - November 2008 - Special Contributors and Sponsors
Food Protection Trends - November 2008 - 872
Food Protection Trends - November 2008 - 873
Food Protection Trends - November 2008 - Call for Awards - IAFP 2009
Food Protection Trends - November 2008 - 875
Food Protection Trends - November 2008 - New Members
Food Protection Trends - November 2008 - 877
Food Protection Trends - November 2008 - 878
Food Protection Trends - November 2008 - 879
Food Protection Trends - November 2008 - What's Happening in Food Safety
Food Protection Trends - November 2008 - 881
Food Protection Trends - November 2008 - 882
Food Protection Trends - November 2008 - 883
Food Protection Trends - November 2008 - 884
Food Protection Trends - November 2008 - Industry Products
Food Protection Trends - November 2008 - 886
Food Protection Trends - November 2008 - 887
Food Protection Trends - November 2008 - 888
Food Protection Trends - November 2008 - Coming Events
Food Protection Trends - November 2008 - 890
Food Protection Trends - November 2008 - Advertising Index
Food Protection Trends - November 2008 - Journal of Food Protection Table of Contents
Food Protection Trends - November 2008 - 893
Food Protection Trends - November 2008 - Audiovisual Library Order Form
Food Protection Trends - November 2008 - Booklet Order Form
Food Protection Trends - November 2008 - Membership Application
Food Protection Trends - November 2008 - Cover3
Food Protection Trends - November 2008 - Cover4
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