Food Protection Trends - November 2008 - 830

James Withee described the use of random sampling; risk-based sampling algorithm and statistical/computational design to sample federally inspected ground beef for the presence of Escherichia coli O157:H7 by the Food Safety and Inspection Service (FSIS). Arthur Miller presented on “Risk Assessment for Thermal Inactivation of Salmonella spp. in Fresh Pork” showing that Salmonella spp. growth can exceed 6 log CFU/g in pork, due to extreme temperature abuses, and during storage/ refrigeration. However, cooking at temperature of 145°F for 15 s, did not increase risk of salmonellosis and at 160°F Salmonella spp. did not survive thermal inactivation. Deon Mahoney described that Salmonella contamination of eggs could be the result of external (surface) or internal (vertical or horizontal transmission). Risk factors include the consumption of uncooked foods containing eggs, use of dirty eggs and concern of food handling. Min Li spoke on the use of predictive modeling of Listeria monocytogenes to monitor the reduction on Fully-Cooked Chicken Drumettes during PostProcess Hot Water Pasteurization. He showed that Weibull model provides a reliable prediction of thermal inactivation of Listeria monocytogenes and post package hot water is effective in reducing the pathogen. George-John Nychas presented a predictive model developed for the growth of Listeria monocytogenes in vanilla cream (traditional milk product). He outlined that Barayni and Roberts model was used of the primary model. The values of the performance indices indicated good fit of the model. Silvia De Lamo-Castellvi assessed the combined Effects of Sucrose Laurate Ester and Pressure-Assisted Thermal Processing to Inactivate Bacillus amyloliquefaciens Spores Suspended in Mashed Carrots. Balasubramanium discussed the combined effect of sucrose laurate ester and high pressure processing to inactivate the Bacillus amyloliquefaciens spores suspended in mashed carrots. He reported the synergistic effect between the two treatments in spore lethality but indicated limited effectiveness of sucrose laurate ester in inhibiting the recovery of remaining spore population. Eelco Franz discussed the role of nutrients and biodiversity in controlling E. coli O157:H7 in the primary production chain of lettuce. He highlighted factors such as fiber content, pH, dissolved organic carbon etc. in controlling the growth of pathogen. Michael Cahn discussed the effect of irrigation on survival and dispersal of surrogate pathogen. He highlighted the effect of applied volume on the recovery rate. Yaguang Luo discussed the effect of abuse of temperature on the safety of the commercially packaged fresh cut salads. AnneMarie Buchholtz gave a presentation on the use of Glo Germ in evaluating the cross contamination during the processing of leafy greens. Carol D’Lima discussed the results for the evaluation of the sensitivity and specificity of Rapid Test Kits for the detection of E. coli O157:H7 from lettuce and leafy greens. t5 – Applied laboratory Methods and novel laboratory Methods technical Session Mawill Rodríguez-Marval, Colorado State University and Saurabh Kumar, University of Nebraska-Lincoln Erin Wenke evaluated different stomaching systems for their effect on viable cells and coliform counts as well as noise levels. Results showed no difference regarding microbial but significant differences were found in terms of noise levels and ease of cleaning. Thomas Romick presented a study evaluating a total bacterial count assay by different collaborating laboratories, using quantitative real time PCR. Results indicated that all labs prepared the standard curve properly, but some variation was observed with the unknown samples. The system is portable, and has a total running time of only 30 minutes. Faith Critzer presented her work on the construction of internal amplification control (IAC) for Salmonella detection using real time RT-PCR. The system helps in eliminating false negatives during assay due to PCR inhibitors. The assay showed a detection limit of 2 log CFU/ml of Salmonella. A system to simplify real time PCR applications in food and environmental testing by minimizing sample preparation was presented by Robert Tebbs. He described a master mix which is resistant to high levels of contaminants, and is cost and time effective. Ebot Tabe presented an evaluation of fecal DNA purification methods and conventional culture methods for detection of E. coli O157:H7 in naturally infected feedlot cattle. The accuracy of detection by QIAmp DNA Stool tests was higher for enriched samples as compared to those non-enriched. Ji-Yeon Hyeon research evaluated VIDAS™ and real time PCR for E. coli O157:H7 detection on inoculated ground beef and radish sprout, by comparing them to a standard culture method. RTPCR and VIDAS™ were superior to culture methods for samples with high numbers of background flora. So-Ri Han compared the sensitivity of KOBAM methods (Korean Food Code Bacterial Manual, KFDA), in order to develop an effective validation system for rapid detection methods of L. monocytogenes. The study highlighted that longer enrichment period is required for proper detection by KFDA method. 830 FOOD PROTECTION TRENDS | NOVEMBER 2008

Food Protection Trends - November 2008

Table of Contents for the Digital Edition of Food Protection Trends - November 2008