Food Protection Trends - January 2010 - 26

TABLE 1. Phages and their host strains used in this study Phage Cb1/204a Cb1/342a BYM Ib3b a b b Phage type Temperate Temperate Virulent Virulent Virulent Host strain L. delbrueckii subsp. lactis 204c L. delbrueckii subsp. bulgaricus342c L. delbrueckii subsp. lactis YSD Vd L. delbrueckii subsp. lactis Ab1d L. delbrueckii subsp. bulgaricus Ib3d YABb isolated from commercial strain L. delbrueckii subsp. lactis Cb1 isolated from a failed manufacture of yogurt c wild strain isolated from natural whey starters d commercial strain FIGURE 1. Lysis plaques obtained for phage Cb1/204, using an inoculum of its host strain (Lactobacillus delbrueckii subsp. lactis 204) obtained from RSM diluted in MRS broth (DO560nm = 0.70) and from MRS overnight diluted (DO560nm = 1) and incubated at 34°C (A, B), 37°C (C, D) and 42°C (E, F) bacillus delbrueckii bacteriophages, either virulent or temperate ones. In addition, growth temperature can influence the characteristics of the plaques, especially if the burst size is low. For this reason, it could be appropriate to incubate the host cells at suboptimal temperatures, providing each phage-strain system the most favorable conditions for plaque enumeration. The aim of this study was to establish the best conditions for enumeration of Lactobacillus delbrueckii phages in order to optimize their counts. MATERIALS AND METHODS Bacterial strains and bacteriophages FIGURE 2. Lysis plaques obtained for phage Cb1/342, using an inoculum of its host strain (Lactobacillus delbrueckii subsp. bulgaricus 342) obtained from RSM diluted in MRS broth (OD 560nm = 0.70) and from MRS overnight diluted (DO560nm = 1) and incubated at 34°C (A, B), 37°C (C, D) and 42°C (E, F) Phages and their host strains used in this study are shown in Table 1. They were all isolated at INLAIN (Instituto de Lactología Industrial, Santa Fe, Argentina) from defective industrial processes of fermented milks (virulent phages Ib3, YAB and BYM) or by induction with mitomycin C (temperate phages Cb1/204 and Cb1/342) (16). Lactobacillus delbrueckii strains were grown and routinely reactivated overnight (42°C) in deMan Rogosa Sharpe (MRS) broth (Biokar, Beauvois, France). They were maintained as frozen (-80°C) stocks in sterile reconstituted (10% wt/vol) commercial nonfat dry skim milk (RSM). Phage stocks were prepared as described by Neviani et al. (9) and stored at 4°C (MRS broth) and -80°C (MRS broth with 15% vol/vol of glycerol). 26 FOOD PROTECTION TRENDS | JANUARY 2010

Food Protection Trends - January 2010

Table of Contents for the Digital Edition of Food Protection Trends - January 2010

Food Protection Trends - January 2010
Contents
A Note from the FPT Scientific Editor
Sustaining Members
Vickie’s View from Your President
Commentary from the Executive Director
Inactivation of Listeria Monocytogenes During Reheating of Frankfurters with Hot Water Before Consumption
Optimization of Methodology to Enumerate Lactobacillus delbrueckii Phages
Highlights from the China International Food Safety & Quality Conference + Expo 2009
Highlights from the Executive Board Meeting
FPT Instructions for Authors
Award Nominations
New Members
What’s Happening in Food Safety
Industry Products
Coming Events
Advertising Index
Journal of Food Protection Table of Contents
Audiovisual Library Order Form
Booklet Order Form
Membership Application
Food Protection Trends - January 2010 - Food Protection Trends - January 2010
Food Protection Trends - January 2010 - Cover2
Food Protection Trends - January 2010 - 1
Food Protection Trends - January 2010 - Contents
Food Protection Trends - January 2010 - A Note from the FPT Scientific Editor
Food Protection Trends - January 2010 - 4
Food Protection Trends - January 2010 - 5
Food Protection Trends - January 2010 - 6
Food Protection Trends - January 2010 - 7
Food Protection Trends - January 2010 - 8
Food Protection Trends - January 2010 - Sustaining Members
Food Protection Trends - January 2010 - 10
Food Protection Trends - January 2010 - 11
Food Protection Trends - January 2010 - Vickie’s View from Your President
Food Protection Trends - January 2010 - 13
Food Protection Trends - January 2010 - Commentary from the Executive Director
Food Protection Trends - January 2010 - 15
Food Protection Trends - January 2010 - Inactivation of Listeria Monocytogenes During Reheating of Frankfurters with Hot Water Before Consumption
Food Protection Trends - January 2010 - 17
Food Protection Trends - January 2010 - 18
Food Protection Trends - January 2010 - 19
Food Protection Trends - January 2010 - 20
Food Protection Trends - January 2010 - 21
Food Protection Trends - January 2010 - 22
Food Protection Trends - January 2010 - 23
Food Protection Trends - January 2010 - 24
Food Protection Trends - January 2010 - Optimization of Methodology to Enumerate Lactobacillus delbrueckii Phages
Food Protection Trends - January 2010 - 26
Food Protection Trends - January 2010 - 27
Food Protection Trends - January 2010 - 28
Food Protection Trends - January 2010 - 29
Food Protection Trends - January 2010 - Highlights from the China International Food Safety & Quality Conference + Expo 2009
Food Protection Trends - January 2010 - 31
Food Protection Trends - January 2010 - Highlights from the Executive Board Meeting
Food Protection Trends - January 2010 - FPT Instructions for Authors
Food Protection Trends - January 2010 - 34
Food Protection Trends - January 2010 - 35
Food Protection Trends - January 2010 - 36
Food Protection Trends - January 2010 - 37
Food Protection Trends - January 2010 - Award Nominations
Food Protection Trends - January 2010 - 39
Food Protection Trends - January 2010 - New Members
Food Protection Trends - January 2010 - 41
Food Protection Trends - January 2010 - What’s Happening in Food Safety
Food Protection Trends - January 2010 - 43
Food Protection Trends - January 2010 - 44
Food Protection Trends - January 2010 - 45
Food Protection Trends - January 2010 - 46
Food Protection Trends - January 2010 - 47
Food Protection Trends - January 2010 - Industry Products
Food Protection Trends - January 2010 - 49
Food Protection Trends - January 2010 - 50
Food Protection Trends - January 2010 - Coming Events
Food Protection Trends - January 2010 - Advertising Index
Food Protection Trends - January 2010 - Journal of Food Protection Table of Contents
Food Protection Trends - January 2010 - Audiovisual Library Order Form
Food Protection Trends - January 2010 - Booklet Order Form
Food Protection Trends - January 2010 - Membership Application
Food Protection Trends - January 2010 - Cover3
Food Protection Trends - January 2010 - Cover4
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