FIGURE 2. Microscopic fluorescent images, at 100× magnification, of stainless-steel coupons contaminated with biofilm, subjected to species/treatment conditions, and subsequently dyed using erythrosin B. (a) Pseudomonas putida/NT; (b) Listeria innocua/NT; (c) Listeria innocua /Enzyme. FIGURE 3. Fluorescence spectra of stainless-steel coupons contaminated with biofilm, subjected to species/treatment conditions, and subsequently dyed using erythrosin B. although on a scale that is currently impractical as a method of assessing hygiene in an industrial setting. While not practical as a rapid or cost-effective method for visualizing the effectiveness of biofilm removal, the fluorescence characteristics of erythrosin B can provide added information to complement visual studies in a laboratory setting. CONCLUSIONS Erythrosin B, is a food grade dye that is effective in enhancing the visualization of biofilm components, is useful in laboratory studies evaluating the effectiveness of cleaner/sanitizer treatments in removing biofilm and other contaminants and has the potential to be developed November/December Food Protection Trends 447