Food Protection Trends - March/April 2023 - 134
available sanitizer performance on conveyor belt material
and reported that when sanitizer concentration, the number
of follow-up treatments, exposure time, and temperatures
were increased over the typical standard daily cleaning and
sanitizing process recommended by the manufacturers,
L. monocytogenes biofilm removal improved from a maximum
1.8-log reduction to a >5.5-log reduction.
Because chemical sanitizers are most commonly used
to reduce microbes on produce harvesting and packing
equipment, the correct selection and use of sanitizing agents
is vital for preventing cross-contamination of product during
harvesting and field-packing operations. Chemical sanitizing
agents approved for use on food-contact surfaces include
halogens (e.g., chlorine- and iodine-based products), ozone,
peroxides (e.g., peracetic acid [PAA]), and quaternary
ammonium compounds (QACs) (15, 141). Although
some researchers have reported that for QACs, higher
minimum inhibitory concentration are needed to kill some
L. monocytogenes strains (51, 160), these strains appear to
be killed at equal efficacy as those killed by lower MICs
(16). Although these findings support the need for use of
appropriate sanitizer concentrations, current evidence is not
sufficient to advise against the use of QAC-based sanitizers.
In numerous studies of sanitizer efficacy against foodborne
pathogens under various conditions (frequently in the
presence of food residues), sanitizer efficacy depended on
the target microorganisms (73, 89, 90, 93, 140, 183). Many
researchers have investigated sanitizer efficacy against
L. monocytogenes, which is a key environmental pathogen due
to its ability to readily attach to surfaces and the frequency at
which it has been identified as both a transient and resident
contaminant in food pro-cessing facilities (26, 112, 152,
156). The efficacy of sanitizers (e.g., PAA, QACs, chlorine, or
chlorine dioxide) commonly used against L. monocytogenes
biofilms has been variable, depending on the materials being
investigated, the specific L. monocytogenes strain, and the
degree of biofilm develop-ment (34, 73, 79, 90). However,
researchers have consistently emphasized the importance of
timely and thorough cleaning of food-contact surfaces before
sanitation to further optimize sanitizer efficacy and reduce
biofilm formation.
Biofilm control
A finding common to many studies is that pathogens
attached to equipment, especially in the form of biofilms,
are much more difficult to eliminate than those present as
individual cells (60, 90, 140, 183). As they mature, biofilms
become more difficult to remove (54, 90, 183). L. monocytogenes
may not form true biofilms but often co-occurs with
biofilm producers such as Pseudomonas and Burkholderia
(159). Korany et al. (90) reported that the efficacies of
QACs, chlorine, chlorine dioxide, and PAA were much lower
against L. monocytogenes cells that have been attached for
7 days than against cells attached for only 2 days.
134 Food Protection Trends March/April
Driven largely by the food manufacturing, meat, dairy, and
medical industries, research into new sanitizing technologies
and/or chemicals with enhanced efficacy against biofilms
has increased over the past decade. Several publications
have provided comprehensive reviews of these innovative
and promising approaches to sanitation, which include use
of visible and UV light, proteins, cold plasma and plasmagenerated
compounds, electric currents, natural products,
nanoparticles, nanomaterials, nanobubbles, peptides,
bacteriophages, and low-frequency high-intensity ultrasound
(5, 6, 42, 67, 114, 121, 138, 144, 175). The mechanisms of
action behind these novel approaches to sanitation are based
on increasing the bacterial cell membrane permeability,
disrupting bacterial communication via quorum sensing
(quorum quenching), promoting bacterial cell lysis,
degrading components of the biofilm matrix, and/or
inhibiting enzyme activity or protein synthesis-all of these
mechanisms may facilitate the disruption and dismantling
of biofilms or bacterial cell death (114, 138, 144).
A hurdle approach to biofilm control or elimination by use
of a combination of sanitizing treatments or technologies
has frequently been more effective against biofilms than a
single antimicrobial intervention alone (114, 132, 138, 174).
For example, ultrasound treatment plus 30 s in 1 ppm of
chlorinated water reduced E. coli O157:H7 counts below the
limit of detection (1.10 CFU/cm2
) on the coring ring blade
and welding joint of lettuce coring knives (188). Rafeeq and
Ovissipour (132) reported a synergistic effect of ultrasound
and nanobubbles against E. coli O157:H7 and Listeria innocua
on spinach leaves that surpassed the efficacy of either
treatment alone. In other cases, bacteriophage addition or
treatments that dismantle or dissolve the biofilm matrix (e.g.,
quorum quenching) in combination with sanitizing agents
have effectively reduced pathogens in biofilms on food-contact
surfaces (42, 138). However, in the " real world " the
persistence of pathogen communities and biofilms is often
due to contamination in equipment locations that are not
reached by chemicals in niches or harborage sites (112). Use
of specific chemicals targeting biofilms is often ineffective
because the liquid or gaseous chemicals cannot reach the
target organisms in hollow areas. In these cases, removal
of persistent pathogen communities and/or biofilms will
require equipment disassembly and enhanced mechanical
cleaning rather than use of specialized chemicals (23).
Validation and verification of cleaning and sanitizing
processes
In the Validation of Cleaning Processes guide for inspectors
of the pharmaceutical industry, the FDA (168) states " the
test of any validation process is whether scientific data shows
that the system consistently does as expected and produces a
result that consistently meets predetermined specifications. "
In a similar way, for the produce industry, cleaning and
sanitizing procedures as outlined in a harvesting company's
Food Protection Trends - March/April 2023
Table of Contents for the Digital Edition of Food Protection Trends - March/April 2023
Perceptions of Risk, Control, and Responsibility Regarding Food Safety among Consumers in Lebanon
Fresh Produce Harvesting Equipment – A Review of Cleaning and Sanitizing Practices and Related Science
Assessing Physical Attributes Associated with Implementation of Risk Management Practices among Small Strawberry Farms
Evaluating Misinformation on YouTube about Washing Produce Before and During the COVID-19 Pandemic
Minimally Processed Vegetables: Consumer Profile, Consumption Habits, and Perceptions of Microbiological Risk
Beyond the Bio
PDG Highlight
Industry Products
Letter to the Editor
Coming Events
Food Protection Trends - March/April 2023 - Cover1
Food Protection Trends - March/April 2023 - Cover2
Food Protection Trends - March/April 2023 - 105
Food Protection Trends - March/April 2023 - 106
Food Protection Trends - March/April 2023 - 107
Food Protection Trends - March/April 2023 - 108
Food Protection Trends - March/April 2023 - 109
Food Protection Trends - March/April 2023 - 110
Food Protection Trends - March/April 2023 - 111
Food Protection Trends - March/April 2023 - Perceptions of Risk, Control, and Responsibility Regarding Food Safety among Consumers in Lebanon
Food Protection Trends - March/April 2023 - 113
Food Protection Trends - March/April 2023 - 114
Food Protection Trends - March/April 2023 - 115
Food Protection Trends - March/April 2023 - 116
Food Protection Trends - March/April 2023 - 117
Food Protection Trends - March/April 2023 - 118
Food Protection Trends - March/April 2023 - 119
Food Protection Trends - March/April 2023 - 120
Food Protection Trends - March/April 2023 - 121
Food Protection Trends - March/April 2023 - 122
Food Protection Trends - March/April 2023 - 123
Food Protection Trends - March/April 2023 - 124
Food Protection Trends - March/April 2023 - 125
Food Protection Trends - March/April 2023 - Fresh Produce Harvesting Equipment – A Review of Cleaning and Sanitizing Practices and Related Science
Food Protection Trends - March/April 2023 - 127
Food Protection Trends - March/April 2023 - 128
Food Protection Trends - March/April 2023 - 129
Food Protection Trends - March/April 2023 - 130
Food Protection Trends - March/April 2023 - 131
Food Protection Trends - March/April 2023 - 132
Food Protection Trends - March/April 2023 - 133
Food Protection Trends - March/April 2023 - 134
Food Protection Trends - March/April 2023 - 135
Food Protection Trends - March/April 2023 - 136
Food Protection Trends - March/April 2023 - 137
Food Protection Trends - March/April 2023 - 138
Food Protection Trends - March/April 2023 - 139
Food Protection Trends - March/April 2023 - 140
Food Protection Trends - March/April 2023 - 141
Food Protection Trends - March/April 2023 - 142
Food Protection Trends - March/April 2023 - 143
Food Protection Trends - March/April 2023 - Assessing Physical Attributes Associated with Implementation of Risk Management Practices among Small Strawberry Farms
Food Protection Trends - March/April 2023 - 145
Food Protection Trends - March/April 2023 - 146
Food Protection Trends - March/April 2023 - 147
Food Protection Trends - March/April 2023 - 148
Food Protection Trends - March/April 2023 - 149
Food Protection Trends - March/April 2023 - 150
Food Protection Trends - March/April 2023 - 151
Food Protection Trends - March/April 2023 - 152
Food Protection Trends - March/April 2023 - 153
Food Protection Trends - March/April 2023 - 154
Food Protection Trends - March/April 2023 - 155
Food Protection Trends - March/April 2023 - 156
Food Protection Trends - March/April 2023 - 157
Food Protection Trends - March/April 2023 - 158
Food Protection Trends - March/April 2023 - 159
Food Protection Trends - March/April 2023 - 160
Food Protection Trends - March/April 2023 - Evaluating Misinformation on YouTube about Washing Produce Before and During the COVID-19 Pandemic
Food Protection Trends - March/April 2023 - 162
Food Protection Trends - March/April 2023 - 163
Food Protection Trends - March/April 2023 - 164
Food Protection Trends - March/April 2023 - 165
Food Protection Trends - March/April 2023 - 166
Food Protection Trends - March/April 2023 - Minimally Processed Vegetables: Consumer Profile, Consumption Habits, and Perceptions of Microbiological Risk
Food Protection Trends - March/April 2023 - 168
Food Protection Trends - March/April 2023 - 169
Food Protection Trends - March/April 2023 - 170
Food Protection Trends - March/April 2023 - 171
Food Protection Trends - March/April 2023 - 172
Food Protection Trends - March/April 2023 - 173
Food Protection Trends - March/April 2023 - 174
Food Protection Trends - March/April 2023 - 175
Food Protection Trends - March/April 2023 - 176
Food Protection Trends - March/April 2023 - 177
Food Protection Trends - March/April 2023 - 178
Food Protection Trends - March/April 2023 - 179
Food Protection Trends - March/April 2023 - Beyond the Bio
Food Protection Trends - March/April 2023 - 181
Food Protection Trends - March/April 2023 - 182
Food Protection Trends - March/April 2023 - PDG Highlight
Food Protection Trends - March/April 2023 - 184
Food Protection Trends - March/April 2023 - 185
Food Protection Trends - March/April 2023 - 186
Food Protection Trends - March/April 2023 - 187
Food Protection Trends - March/April 2023 - Industry Products
Food Protection Trends - March/April 2023 - 189
Food Protection Trends - March/April 2023 - 190
Food Protection Trends - March/April 2023 - 191
Food Protection Trends - March/April 2023 - 192
Food Protection Trends - March/April 2023 - Letter to the Editor
Food Protection Trends - March/April 2023 - 194
Food Protection Trends - March/April 2023 - 195
Food Protection Trends - March/April 2023 - 196
Food Protection Trends - March/April 2023 - 197
Food Protection Trends - March/April 2023 - 198
Food Protection Trends - March/April 2023 - 199
Food Protection Trends - March/April 2023 - Coming Events
Food Protection Trends - March/April 2023 - Cover3
Food Protection Trends - March/April 2023 - Cover4
https://www.nxtbook.com/nxtbooks/trilix/fpt_20241112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20240910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20240708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20240506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20240304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20240102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20231112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20230910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20230708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20230506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20230304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20230102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20221112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20220910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20220708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20220506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20220304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20220102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20211112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20210910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20210708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20210506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20210304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20210102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20201112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20200910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20200708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20200506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20200304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20200102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20191112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20190910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20190708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20190506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20190304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20190102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20181112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20180910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20180708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20180506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20180304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20180102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20171112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20170910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20170708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20170506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20170304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20170102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20161112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20160910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20160708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20160506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20160304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20160102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20151112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20150910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20150708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20150506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20150304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20150102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20141112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20140910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20140708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20140506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20140304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20140102
https://www.nxtbook.com/nxtbooks/trilix/fpt_20131112
https://www.nxtbook.com/nxtbooks/trilix/fpt_20130910
https://www.nxtbook.com/nxtbooks/trilix/fpt_20130708
https://www.nxtbook.com/nxtbooks/trilix/fpt_20130506
https://www.nxtbook.com/nxtbooks/trilix/fpt_20130304
https://www.nxtbook.com/nxtbooks/trilix/fpt_20130102
https://www.nxtbook.com/nxtbooks/trilix/fpt_201212
https://www.nxtbook.com/nxtbooks/trilix/fpt_201211
https://www.nxtbook.com/nxtbooks/trilix/fpt_201210
https://www.nxtbook.com/nxtbooks/trilix/fpt_201209
https://www.nxtbook.com/nxtbooks/trilix/fpt_201208
https://www.nxtbook.com/nxtbooks/trilix/fpt_201207
https://www.nxtbook.com/nxtbooks/trilix/fpt_201206
https://www.nxtbook.com/nxtbooks/trilix/fpt_201205
https://www.nxtbook.com/nxtbooks/trilix/fpt_201204
https://www.nxtbook.com/nxtbooks/trilix/fpt_201203
https://www.nxtbook.com/nxtbooks/trilix/fpt_201202
https://www.nxtbook.com/nxtbooks/trilix/fpt_201201
https://www.nxtbook.com/nxtbooks/trilix/fpt_201112
https://www.nxtbook.com/nxtbooks/trilix/fpt_201111
https://www.nxtbook.com/nxtbooks/trilix/fpt_201110
https://www.nxtbook.com/nxtbooks/trilix/fpt_201109
https://www.nxtbook.com/nxtbooks/trilix/fpt_201108
https://www.nxtbook.com/nxtbooks/trilix/fpt_201107
https://www.nxtbook.com/nxtbooks/trilix/fpt_201106
https://www.nxtbook.com/nxtbooks/trilix/fpt_201105
https://www.nxtbook.com/nxtbooks/trilix/fpt_201104
https://www.nxtbook.com/nxtbooks/trilix/fpt_201103
https://www.nxtbook.com/nxtbooks/trilix/fpt_201102
https://www.nxtbook.com/nxtbooks/trilix/fpt_201101
https://www.nxtbook.com/nxtbooks/trilix/fpt_201012
https://www.nxtbook.com/nxtbooks/trilix/fpt_201011
https://www.nxtbook.com/nxtbooks/trilix/fpt_201010
https://www.nxtbook.com/nxtbooks/trilix/fpt_201009
https://www.nxtbook.com/nxtbooks/trilix/fpt_201008
https://www.nxtbook.com/nxtbooks/trilix/fpt_201007
https://www.nxtbook.com/nxtbooks/trilix/fpt_201006
https://www.nxtbook.com/nxtbooks/trilix/fpt_201005
https://www.nxtbook.com/nxtbooks/trilix/fpt_201004
https://www.nxtbook.com/nxtbooks/trilix/fpt_201003
https://www.nxtbook.com/nxtbooks/trilix/fpt_201002
https://www.nxtbook.com/nxtbooks/trilix/fpt_201001
https://www.nxtbook.com/nxtbooks/trilix/fpt_200912
https://www.nxtbook.com/nxtbooks/trilix/fpt_200911
https://www.nxtbook.com/nxtbooks/trilix/fpt_200910
https://www.nxtbook.com/nxtbooks/trilix/fpt_200909
https://www.nxtbook.com/nxtbooks/trilix/fpt_200908
https://www.nxtbook.com/nxtbooks/trilix/fpt_200907
https://www.nxtbook.com/nxtbooks/trilix/fpt_200906
https://www.nxtbook.com/nxtbooks/trilix/fpt_200905
https://www.nxtbook.com/nxtbooks/trilix/fpt_200904
https://www.nxtbook.com/nxtbooks/trilix/fpt_200903
https://www.nxtbook.com/nxtbooks/trilix/fpt_200902
https://www.nxtbook.com/nxtbooks/trilix/fpt_200901
https://www.nxtbook.com/nxtbooks/trilix/fpt_200812
https://www.nxtbook.com/nxtbooks/trilix/fpt_200811
https://www.nxtbook.com/nxtbooks/trilix/fpt_200810
https://www.nxtbook.com/nxtbooks/trilix/fpt_200809
https://www.nxtbook.com/nxtbooks/trilix/fpt_200808
https://www.nxtbook.com/nxtbooks/trilix/fpt_200807
https://www.nxtbookmedia.com