Magnetics Business & Technology - September/October 2021 - 13

FEATURE ARTICLE
distribution and reduces toxicity. This allows reducing the required
nucleic acid or virus doses and the process time of delivery which
is crucial for improvement of in vivo nucleic acid delivery.
Their association with the gene
vectors such as DNA, siRNA,
ODN or virus is achieved by saltinduced
colloidal aggregation and
electrostatic interaction. The magnetic
particles are then concentrated
onto cells by the influence of an
external magnetic field generated
by a specific magnetic plate. The
cellular uptake of the genetic material
is accomplished by endocytosis
and pinocytosis, two natural
biological processes.
individual magnets and Mega Magnetic Plate.
Another tool is a magnetic separation
rack that holds various sizes of
standard laboratory tubes. It is specifically
designed for capture, concentration,
washing and elution when
using the company's Mag4C technology.
Mag4C magnetic nanoparticles
capture viruses in culture medium by
electrostatic and hydrophobic interactions
with 80-99 % efficiency. Once captured onto magnetic beads,
viruses can be concentrated and stored with a conservation buffer
or directly used for cell culture, molecular biology or other assays.
Magnetic separation rack
In vivo magnets set
Complementary to the reagents is an in vivo magnet set that includes
three cylinders ranging from 2mm to 10mm and a square
magnet sized 18mm by 18 mm. The variations accommodate positioning
either internally for deep organs or focalized gene transfer,
or externally for large or isolated organs such as liver, brain,
muscle, subcutaneous tumor.
NeuroMag
Positioned as a versatile and universal
technology adapted to all types of
nucleic acids and viruses, the Magnetofection
platform has evolved to a
broad range of reagents designed according
to defined applications. A few
examples are NeuroMag for neurons
transfection, ViroMax and AdenoMag
for various viruses, FluoMag for fluorescent
requirements, PolyMag and
LipoMag for various nucleic acids.
NeuroMag transfection reagent is designed
specifically for neurons from 1 DIV to 21 DIV. It has proven
to be extremely efficient in transfecting a large variety of primary
neurons such as cortical, hippocampal, dorsal root ganglion and
motor neurons with all types of nucleic acids. Due to its unique
properties, NeuroMag allows following transfected neurons during
several days.
PolyMag CRISPR kit is a magnet assisted
transfection reagent optimized to
deliver high level of plasmid DNA and
varieties of RNA for gene editing experiments,
providing high transfection efficiency
without toxicity.
PolyMag CRISPR
OZ Biosciences has also developed
various assay kits and tools for its systems.
The only equipment requirement for standard Magnetofection
is a specially designed reusable magnetic plate since the
technology requires an appropriate,
heterogenous magnetic field that magnetizes
the nanoparticles in solution,
forms a very strong gradient to attract
the nanoparticles onto cells and covers
all the surface of the culture dish. Three
magnetic plates are available: Super
Magnetic Plate, Magnetic Plate with 96
96-well magnetic plate
www.MagneticsMag.com
September/October 2021 * Magnetics Business & Technology 13
Headquarters of the firm are at the
Luminy Scientific Park near Marseille,
close to a cluster of academic
and commercial biological research
activities. It also has a branch operation
in San Diego, CA. The company
was founded by three internationally
recognized experts in molecular delivery systems, gene therapy
and vaccines: Olivier Zelphati, the CEO; Christian Plank and Jean
Haensler, who both serve as scientific advisors. Over its 17 years,
it has received several prestigious national and international
awards, and numerous government grants helping to fund its research.
For
more info, see www.ozbiosciences.com.
http://eee.ozbiosciences.com http://www.elytone.com.tw http://www.MagneticsMag.com
Magnetics Business & Technology - September/October 2021

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