Tumori Journal Abstract Book - October 2020 - 159

N - Head and Neck Tumours	
longitudinal changes were correlated with outcome.
P<0.05 was considered for statistical significance.
Results: 16 patients were analyzed. Longitudinal analysis
showed that CXCL-10, TNF-α and IFN-γ increased from
T0 to T3 (P=0.0233; P=0.049 and P=0.035, respectively).
IL-15 increased from T0 to T2 (P=0.024), but decreased at
PD (P=0.024). No other significant longitudinal changes
were observed. Considering the basal values, we used
ROC analysis to cluster all patients in two groups, observing a better PFS in those whose T0 values of IL-5, IL-6 and
IL-10 were below than the respective cut-off points (HR
0.12, P=0.01; HR 0.056, P=0.008; HR 0.055, P=0.009) but
only IL-5 below the cut-off positively correlated with OS
(HR 0.05, P=0.007). We could not perform multivariate
analysis due to the limited number of events. Therefore we
use PCA analysis to group patients with good or poor PFS
using T0 levels of IL-5, IL-6, IL-10, CCL-22 (KMO 0.574,
P=0.025); the same analysis allowed to clusterize patients
with OS above or below median value using T0 levels of
IL-5, IL-6, IL-10, IL-15 (KMO 0.636, P<0.001).
Conclusions: Our results show that nivolumab therapy
could increase the levels of IL-15. CXCL-10, TNF-α and
IFN-γ increased at PD. Moreover our data suggest that
IL-5, IL-6 and IL-10 might predict the benefit from the
treatment.

N05
MYB-rearranged and nonrearranged adenoid cystic
carcinoma: are there any clinical
differences?
Filippini D.M.1, Cavalieri S.2, Quattrone P.2, Bergamini C.2, Resteghini
C.2, Colombo E.2, Platini F.2, Alfieri S.2, Locati L.2, Licitra L.2
1
IRCCS Istituto Nazionale dei Tumori, Policlinico Sant' Orsola Malpighi,
Milano, Bologna; 2IRCCS Istituto Nazionale dei Tumori, Milano

Background: Adenoid cystic carcinoma (ACC) is the second most common salivary gland carcinoma (SGC) subtype. MYB/MYBL1 rearrangements have been detected in
more than 60% of cases. The impact of these rearrangements on clinical behaviors is still unclear.
Material (Patients) and Methods: This is a retrospective
monocentric observational study. Both salivary gland and
extra salivary ACC patients (pts) tested for MYB rearrangement have been included. Demographic characteristics, histological pattern and disease stage at diagnosis
were analyzed. Differences in clinical and pathological
characteristics were compared between MYB rearranged
(MYB+) and non-rearranged (MYB-) ACC pts. MYB
rearrangements were tested by FISH.
Results: From 2006 to 2019 48 ACC pts have been identified. Median follow-up was 7.8 years (y) (95%CI 5.4-9.8).
Thirty-three pts (13 males) were MYB+ and 15 (9 males)

159
MYB-. Median age in the MYB+ group was 48 y ( 27-73)
while in MYB- it was 51 (21-77). Histologically, the solid
pattern was mainly associated with MYB+ (N 13, 40%)
while the tubular- cribriform pattern was mainly represented in MYB- (N10, 66%), p=0.097. Data about clinical
history were available in 44 out 48 pts. At initial diagnosis,
36 pts (26 MYB+ vs 10 MYB-) had local or loco-regional
disease, and 8 pts (4 MYB+ vs 4 MYB -) had metastatic
disease (lung metastases only). Four pts (2 MYB+, 2
MYB-) were alive and disease-free at last follow-up, 12
pts relapsed locally/locoregionally (in MYB + only,
p-value 0.018), and 28 pts (16 MYB+ and 12 MYB-)
relapsed at distant sites. The most common metastatic site
was lung. Time to progression requiring active treatment
was 55 and 12 months in MYB+ and MYB-, respectively.
In 16 pts (33%), genome sequencing data were available:
single mutations of KIT (7%), TP53 (7%), and MET (15%)
genes were found in MYB+, a PIK3CA mutation in 1
MYB- was discovered. In 1/6 MYB+ pts microsatellite
instability (MSI) was found.
Conclusions: In this small cohort of ACC pts (MYB+ vs
MYB-), clinical, pathological and genomic characteristics
seem to identify different ACC subtypes. In particular
loco-regional recurrence has been associated only to
MYB+. A trend to a higher number of genetic alterations
has been observed in MYB+. Further studies are warranted to deepen the knowledge about the clinical significance of the MYB/MYBL1 rearrangements to better
stratify patients' prognosis.

N06
PROGNOSIS AND MANAGEMENT
PRINCIPLES OF HEAD AND NECK
CANCERS DURING PREGNANCY: A CASE
SERIES
Bergamini C.1, Cavalieri S.1, Locati L.1, Alfieri S.1, Resteghini C.1,
Platini F.1, Colombo E.1, Licitra L.1
1

Fondazione IRCCS Istituto Nazionale dei Tumori, Milano

Background: Pregnancy-associated cancer is a cancer
occurring during pregnancy or within 12 months after it.
Head and neck cancer (HNC) during pregnancy is a rare
phenomenon. The diagnosis of HNC in the gestational
period poses many difficult decisions as multiple clinical,
personal, and ethical factors need to be considered.
Therapeutic approaches need to be tailored to each pregnant woman with HNC.
Methods: This is a retrospective study on women whose
HNC was diagnosed during pregnancy to investigate both
pregnancy and oncological outcomes.
Results: During the period 2005-2019, 15 pregnant
women with HNC were treated at the Istituto Nazionale
Tumori of Milan. Five patients (pts) suffered from salivary
Tumori Journal Abstract Book - October 2020

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