UEGJournal Abstracts 2021 - 377

Nutrition
Conclusion: IBS patients display altered gut microbiota after the SSRD,
with increased beta diversity and specific changes in genera. These findings
highlight the need to consider dietary habits in longitudinal studies
examining human gut microbiota. Further research is needed to establish
if diet-induced alterations in gut microbiota affect symptom burden and
long-term disease development in IBS.
References: 1. Nilholm C, Larsson E, Sonestedt E, Roth B, Ohlsson B. Assessment
of a 4-Week Starch- and Sucrose-Reduced Diet and Its Effects
on Gastrointestinal Symptoms and Inflammatory Parameters among Patients
with Irritable Bowel Syndrome. Nutrients. 2021 Jan 28;13(2):416.
doi: 10.3390/nu13020416. PMID: 33525489; PMCID: PMC7911460.
Disclosure: Nothing to disclose.
P0205
FEEL4DIABETES HEALTHY DIET SCORE APPLIED FOR TYPE 1
DIABETES PATIENTS WITH LONG DURATION IN BULGARIA
L. Dimitrov1, D. Zhelyazkova1
K. Tsochev1
G. Valchev1
, Y. Bocheva1
, Y. Yotov1
, F. Ahmed1
, G. Chausheva1
, V. Iotova1
1Medical University of Varna, Varna, Bulgaria
Contact E-Mail Address: l.d@mail.bg
Introduction: Diet quality indices are mathematical algorithms aimed at
quantifying the degree of adequacy between actual intakes of nutrients
or food groups within population groups. A recently developed Feel4diabetes
healthy diet score (FHDS) is an elegant tool validated in 6 European
countries, including Bulgaria, and helps the assessment of nutrition. Adherence
to healthy nutrition is an integral part of the treatment and selfmanagement
of type 1 diabetes (T1D) patients.
Aims & Methods: The aim of our study is to assess the diet of patients
with T1D with long duration and controls by means of the Feel4Diabetes
healthy diet score.
Methods: The study was conducted in Bulgaria over a period of 2 years
2017-2019. A total of 183 participants were included - 125 patients (68.3%),
52.8% male (n=66); mean age 42.7±10.4years; BMI 25.25±4.03 kg/m2
) with
T1D with long disease duration (mean duration 25.3±8.2years) and 58
controls matched for gender, age and BMI. Dietary habits were assessed
with the application of a semiquantitative food frequency questionnaire,
which re-calculated using the FHDS with a maximum possible score of 100
points (a higher score reflects a healthier diet).
Results: The results show that T1D female patients have significantly better
nutrition measured with the FHDS (62.0±12.9 vs. 54.9±8.8, p=0.04),
which is mainly due the significant difference for the score reflecting presence
of breakfast throughout the week. Male patients do not significantly
differ in their dietary habits from controls - FHDS of 55.0±12.8 vs. 54.4±9.8,
p=0.8. Although in males there was a significant beneficial trend from the
presence of breakfast, it was outweighed by the negative tendency for the
consumption of unhealthy oils, fats and salty snacks.
Conclusion: Female T1D patients demonstrated better dietary habits
compared to controls, while the positive tendencies in males were outweighed
by negative habits. Additional dietary education is needed to improve
eating behaviors in T1D patients.
References: 1. Guerrero ML, Pérez-Rodríguez F, Hueda MC. Diet quality indices
for nutrition assessment: Types and applications. Functional FoodImprove
Health through Adequate Food. 2017 Aug 2;1:283-308.
2. Virtanen E, Kivelä J, Wikström K, Lambrinou CP, De Miguel-Etayo P, Huys
N, Vraukó-Tóth K, Moreno LA, Usheva N, Chakarova N, Rado SA. Feel4Diabetes
healthy diet score: development and evaluation of clinical validity.
BMC endocrine disorders. 2020 May;20:1-0.
Disclosure: Nothing to disclose.
Vol. 9 | October 2021
, N. Usheva1
,
, M. Boyadzhieva1
,
Contact E-Mail Address: claire.plissonneau@uca.fr
Introduction: As current treatments of Crohn's disease (CD, a disease involving
dysbiosis and expansion of mesenteric adipose tissue) are mainly
symptomatic, it is necessary to identify preventive strategies that promote
healthy microbiota and decrease inflammation. Physical activity (PA) is an
efficient strategy for positively influencing gut microbiota and by decreasing
visceral fat mass. Moreover, Western diet, rich in lipids and in n-6 polyunsaturated
fatty acids (PUFAs), is a risk factor of CD onset and leads to gut
microbiota alteration.
Aims & Methods: We aim to evaluate the preventive effect of spontaneous
PA and/or linseed supplementation in two forms (oil or extruded seeds)
rich in n-3 PUFAs (for a n-6 PUFAs/n-3 PUFAs ratio rebalance) using a
mouse model mimicking CD susceptibility.
Transgenic CEABAC10 male mice (8 week-old), expressing human
CEACAM6 which favor adherent invasive E. coli (AIEC) colonization, were
randomized in 4 groups for 12 weeks: CTL (normal diet, n=11), HFD (Highfat
diet; n=15), LS-O (HFD with linseed oil supplementation; n=16) and LS-E
(HFD with extruded linseed supplementation; n=15). Moreover, HFD, LS-O
and LS-E mice performed spontaneous activity using individual wheel (total
distance and speed were recorded during the 12 weeks). Then, mice
were treated with 1% dextran sodium sulfate (DSS) and after 3 days were
orally challenged with the AIEC LF82 strain for a 7 day-period. Body composition,
inflammation, mucosa-associated microbiota composition, LPS
and short-chain fatty acids production were assessed.
Results: After the 12 week-HFD period including or not linseed-supplementation
(oil or extruded linseed), no significant change in whole-body
composition was observed (fat and fat free mass). Concomitantly, total
energy intake and spontaneous PA assessed from wheel activity were not
different between groups. Following the infection of mice with the AIEC
LF82 pathobiont bacteria, no difference was observed in body weight and
amount of visceral fat mass. However, the level of fecal lipocalin 2, which
reflects the level of intestinal inflammation, was negatively correlated
with the level of PA (mean distance/day ; r = -0.4343; p = 0.0301), suggesting
a beneficial impact of PA. In addition, epidydimal adipose tissue KC
secretion as well as plasma LPS levels were significantly reduced in the
two groups receiving n-3 PUFAs diet-supplementation (LS-O and LS-E
groups). Concerning the mucosa-associated gut microbiota on colon samples,
β-diversity in the LS-E group was significantly different compared to
HFD and LS-O group. Moreover, the α-diversity, illustrated by the Shannon
index tended to increase in the LS-E group. The abundance of the specific
features Prevotella, Paraprevotella, Ruminococcus and Clostridiales were
significantly greater in the LS-E compared to HFD and LS-O groups. Functionally,
the level of SCFA, and more specifically butyrate, was increased
with diet supplemented in extruded linseed as a matrix.
Conclusion: PA and n-3 PUFAs diet rebalancing demonstrated beneficial
effects on moderate intestinal and adipose tissue inflammation using a
model mimicking CD susceptibility. In addition, n-3 PUFAs diet rebalanc377
P0206
BENEFICIAL
EFFECTS OF LINSEED DIET-SUPPLEMENTATION AND
SPONTANEOUS PHYSICAL ACTIVITY ON INFLAMMATION AND THE
MUCOSA-ASSOCIATED MICROBIOTA
C. Plissonneau1,2
M. Etienne2, I. Wawrzyniak5
G. Chesneau7
, A. Sivignon1, B. Chassaing3
, P. Chausse6
, N. Boisseau2, N. Barnich1
1Université Clermont Auvergne, M2iSH, Clermont-Ferrand, France,
2Université Clermont Auvergne, AME2P, Clermont-Ferrand, France,
3Inserm U1016, Paris, France, 4INRAE, UNH, Clermont-Ferrand,
France, 5Université Clermont Auvergne, LMGE, Clermont-Ferrand,
France, 6Université Clermont Auvergne, LaPSCo, Clermont-Ferrand,
France, 7Valorex, Combourtillé, France
, F. Capel4
, F. Dutheil6
, V. Martin2
,
,
, G. Mairesse7
UEGJournal Abstracts 2021

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