eBook: Optimizing Gene-Editing Workflows - 20

Table 1. Benefits and drawbacks of pooled and arrayed CRISPR screens.
Cell models can affect screening outcomes
The type of screen one chooses also depends on the
cell model used. Because pooled screens require
the integration of sgRNA into the genome and passage
to daughter cells, they are most appropriate
for use with actively dividing cells. Pooled screens
are thus not well-suited for primary cells and neurons,
which have a limited capacity to proliferate.
Alternatively, arrayed screens do not require an extended
period of expansion and can be used with a
variety of cell types.
Time and labor may vary between the
two formats
The amount of time and labor associated with
each screen workflow is often different. For pooled
screens, plasmid preparation and viral packaging require
considerable upfront work. Also, because cells
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