Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 7

Redefining Efficiency in Biotherapeutic Protein Analysis * Retooling Protein Characterization

Ph.D., principal scientist, Amgen, discussed nanodisc as a reliable and
enabling method for solubilizing and stabilizing IMPs into detergentfree lipid-bilayer environments.
The IMP is stabilized within the nanodisc structure "by a pair of
membrane scaffold proteins and the lipid bilayer, which mimics the
endogenous cellular membrane without detergent interference,"
explained Dr. Xu. "Thus, IMPs are likely to be presented in their native
form or in a form very close to their native form in nanodisc."
"This approach," he continued, "enables a wide range of detection methods such as SPR (surface plasmon resonance), BSI (back scattering interferometry), and AUC (analytical ultracentrifugation) to be employed for
detailed biophysical and biochemical characterization of membranes."
Dr. Xu noted that he had determined binding properties of peptide
and small molecule inhibitors of Kv.1.3. He reported that he had used
a chimeric ion channel, Kcs-Kv.1.3 nanodisc, and direct binding technologies. His results, he asserted, would have been impossible using
micelles or liposomes.
Kv.1.3, an important therapeutic target, is a potassium voltage-gated ion
channel with key roles in regulating activation of immune-response-signaling pathways. Although Kv.1.3 function remains to be fully elucidated,
dysregulation is implicated in chronic inflammatory syndromes. There is
7

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Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook

Table of Contents for the Digital Edition of Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook

Contents
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 1
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 2
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 3
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - Contents
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 5
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 6
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 7
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 8
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 9
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 10
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 11
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 12
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 13
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 14
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 15
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 16
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 17
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 18
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 19
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 20
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 21
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 22
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 23
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 24
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 25
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 26
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 27
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 28
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 29
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 30
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 31
Redefining Efficiency in Biotherapeutic Protein Analysis - PerkinElmer eBook - 32
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