TTP Labtech, Phenotypic Screening - 8

PHENOTYPIC SCREENING an e-Book Series Perspective

Case Study 1
3D culture formats are required to correctly
address the role of the KRAS pathway on
cancer cell growth and survival
Studies at Horizon Discovery Group plc (U.K.) investigated
the effect of a KRAS mutation on the growth rates and drug
sensitivity of a colon cancer cell line grown in 2D and 3D.
While the mutation had no effect on the growth rate of cells in
conventional 2D adherent cultures, cells grown as 3D colonies
in soft agar showed a reduced growth phenotype when the
KRAS mutation was knocked-out (Figure 1). Furthermore,
cells harbouring the KRAS mutation showed enhanced
drug sensitivity when grown in 3D rather than 2D.
Figure 1. The effect of a KRAS mutation on cancer cell growth in
2D and 3D cell cultures

8 | GENengnews.com



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TTP Labtech, Phenotypic Screening

Table of Contents for the Digital Edition of TTP Labtech, Phenotypic Screening

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TTP Labtech, Phenotypic Screening - 1
TTP Labtech, Phenotypic Screening - 2
TTP Labtech, Phenotypic Screening - 3
TTP Labtech, Phenotypic Screening - Contents
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